![]() The specimen employed in this discussion is a brightfield color photomicrograph of a quadruple-stained thin section of alfalfa plant tissue that has been infected with the crown wart fungus, Physoderma alfalfae. ![]() White and highlight regions of the image expose all three emulsion layers equally (or almost so) due to the fact that white light is composed of all three primary colors. ![]() Areas of the image that are black do not expose the emulsion because black does not reflect any of the three primary colors. Wavelengths that fall in between these divisions will react with two or more of the emulsion layers to reproduce that color when light is viewed through the processed film. In most color transparency films, the top layer is sensitive to blue light, while the middle and lower layers are sensitive to green and red light, respectively. Each layer of a color film emulsion absorbs a specific spectrum of visible light wavelengths, prescribed by the properties of the dyes embedded in that layer.
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